K3 camera 更新
K3 Camera／gatan 信息出来了：（网址：http://www.gatan.com/k3-camera）Introducing Gatan’s K3 direct detection camera for cryo-electron microscopy. This true next generation camera is of a completely new design and does not share any parts with the K2. The K3 is the complete and latest expression of Gatan’s deep experience in single electron counting direct detection cameras.[*]Better
[*]Powerful in-line signal processing will raise the DQE beyond that of the K2 camera.
[*]Optional in-line, GPU-based motion correction avoids the need to save terabytes of raw frames.
[*]Larger
[*]24 megapixels (5,760 x 4,092) – 1.6x higher than K2.
[*]Faster
[*]1,500 full frames per second – 3.75x higher than K2.
Even ignoring the benefits from a higher DQE, the productivity of the K3 will be some 6x higher than that of the K2.Insist on the highest performance camera for cryo-EM – the K3. Shipments are expected to start in the third quarter of 2017.The many early adopters who already purchased the K2 camera with a K3 upgrade will receive the GPU option for free as our appreciation for their trust in Gatan.Discover how K3 improves upon the K2 camera and will continue the tradition of breaking boundaries in cryo-EM – year after year.
由K3 引发的讨论【注意：讨论仅代表部分人观点～～大家酌情理解】：
如果不另外耗计算耗存储，official的in-line GPU-based motion correction 能实时跑满1500 frames/s 吗？
答：每个frame的剂量太低，没法准确align，motion correction也就失效了。High frame rate means high electron dose rate. High electron dose rate means …
那么，如果成像速率足够高， 是否可以在样品损伤前，把图像信息收到？
猜想：dose可以变高的话，那么每个frame就算时间很短，也有足够的信息做alignment。这样也许就可以更充分得sample 样品的漂移，也就可能带来更精准的motion correction。
引发：XFEL是如何解决这个问题的？
答：XFEL 是样品快速流过射线源，样品全靠shutter保护。 比较关心的问题，EPU兼容K3 吗？
有人有内部消息吗 http://www.gatan.com/k3-camera
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