postprocessing problem
大家好，我有个Postprocessing problem，3D Classification计算已经到14埃了，但接着做Postprocessing，出现了WARNING，并提示如下，不知是哪里出现了问题，求解( ⊙ o ⊙ )啊！O(∩_∩)O谢谢
== Masking input maps ...
+ randomize phases beyond: 20 Angstroms
WARNING: The phase-randomised FSC is larger than 0.10 at the estimated resolution!
WARNING: This may result in an incorrect resolution estimation. Provide a softer mask with less features to get lower phase-randomised FSCs
3D Classification：_rlnCurrentResolution 14.000000
chimera threshold：0.0037
== Reading input half-reconstructions:
+ half1-map: D1/run_2D_sel_clx_cls3_D1_3DsM_ct20_half1_class001_unfil.mrc
+ half2-map: D1/run_2D_sel_clx_cls3_D1_3DsM_ct20_half2_class001_unfil.mrc
== Perform auto-masking ...
+ density threshold: 0.00405
+ extend ini mask: 5 pixels
+ width soft edge: 5 pixels
== Extending initial binary mask ...
1.03/1.03 min ............................................................~~(,_,">
== Making a soft edge on the extended mask ...
51/51 sec ............................................................~~(,_,">
== Masking input maps ...
+ randomize phases beyond: 20 Angstroms
WARNING: The phase-randomised FSC is larger than 0.10 at the estimated resolution!
WARNING: This may result in an incorrect resolution estimation. Provide a softer mask with less features to get lower phase-randomised FSCs.
== Dividing map by the MTF of the detector ...
+ mtf STAR-file: mtf-k2-200kev.star
== Applying sqrt(2*FSC/(FSC+1)) weighting (as in Rosenthal & Henderson, 2003) ...
== Fitting straight line through Guinier plot to find B-factor ...
+ fit from resolution: 10
+ fit until resolution: 0
+ slope of fit: -135.631
+ intercept of fit: -14.1714
+ correlation of fit: 0.540263
+ apply b-factor of: -542.524
== Low-pass filtering final map ...
+ filter frequency: 12
== Writing out put files ...
+ Processed map: D1/run_2D_sel_clx_cls3_D1_3DsM_ct20_p2.mrc
+ Processed masked map: D1/run_2D_sel_clx_cls3_D1_3DsM_ct20_p2_masked.mrc
+ Auto-mask: D1/run_2D_sel_clx_cls3_D1_3DsM_ct20_p2_automask.mrc
+ Metadata file: D1/run_2D_sel_clx_cls3_D1_3DsM_ct20_p2.star
+ FINAL RESOLUTION: 12
@小雨 你好呀zzh~
Relion的post processing在计算FSC的时候，会加入mask。
为了防止这个mask太紧，过高的估计分辨率，relion会自动生成一个噪音的对照组，加上同样的mask，如果这个mask使得噪音也产生了大于0.10的FSC，就说明你所估计的分辨率，除了信号的相关性之外，还有你自己的mask的相关性。这个时候relion就会warning你~
解决办法是把mask搞得松一些，改变这三个值：
== Perform auto-masking ...
+ density threshold: 0.00405
+ extend ini mask: 5 pixels
+ width soft edge: 5 pixels 小雨 发表于 2017-7-22 16:48
你好呀zzh~
Relion的post processing在计算FSC的时候，会加入mask。
非常感谢！！ zzh8084 发表于 2017-7-22 21:30
非常感谢！！
记得给有营养的帖子,评分哦.:) Zheng_Liu 发表于 2017-8-3 11:24
记得给有营养的帖子,评分哦.
:D 好的，
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